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Title: The role of the macrophage in the immune response
Author: Pugh, Christopher W.
ISNI:       0000 0001 2453 024X
Awarding Body: University of Oxford
Current Institution: University of Oxford
Date of Award: 1981
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In this thesis the mesenteric lymphadenectomised rat model has been validated as a method for collecting cells derived from peripheral lymph draining the intestine. This model has been employed to study the non-lymphoid cells (NIC) of rat peripheral lymph. These cells are heterogeneous with regard to their surface morphology but evidence is presented that surface morphology is a labile marker. NIC have been characterised in terms of their ultrastructure, cytochemistry, surface markers and endocytic capabilities. Evidence is presented suggesting that these cells are of bone marrow origin. Various features of their life history are described including the location of similar cells in the intestinal wall and evidence that NIC turn-over rapidly and do not recirculate. Physical and biological properties of NIC have been used to devise a variety of techniques for separating NIC from the thoracic duct lymphocytes with which they are collected. Conditions have been established that allow the in vitro pro- liferation responses of rat lymphocytes to be studied. Functional properties of NIC as accessory cells in these proliferative responses and as potent stimulators of primary mixed leukocyte reactions are described. Comparisons are made between NIC and other non-lymphoid cells that have been described in various locations in the immune systems of the rat and other species. These comparisons suggest that NLC are related to the dendritic cell described by Steinman and the interdigitating cell described by Veldman. NLC show marked differences from classical macrophages although they may be members of the mononuclear phagocyte system. The relationship between NLC, Langerhans cells and other cells collected from peripheral lymph is discussed. In the light of these comparisons and published data on the functional capabilities of these different types of non-lymphoid cell their probable roles in immune responses are discussed.
Supervisor: MacPherson, Gordon G. Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Macrophages ; Immune response