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Title: Studies on serological variation among bovine rotaviruses
Author: Ojeh, Clement Kanyidinma
ISNI:       0000 0001 3455 6427
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1983
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All 12 field strains of rotavirus used initially to provide virus stock and convalescent antisera were capable of multiplying in gnotobiotic Lambs but without inducing diarrhoea. None grew on primary bovine and ivine embryonic kidney cell cultures nor on MDBK, LLC-MK2 and Vero cell Lines but all adapted well to growth in roller cultures of MA104 cells ireated with lOpg/ml of trypsin. Pre-treatment of virus inocula was essential for further propagation of the virus strains on MA104 cells. Because 4 of 12 original strains were found to contain more than one rotavirus electrophoretype, the remaining 8 strains were selected for more letailed serological investigations. Neutralisation tests using convalescent lamb antisera and hyperimmune rabbit antisera against faecal ind tissue culture viruses confirmed the presence of two distinct serotypes; in that they showed more than a 20-fold reciprocal difference Ln titre and an antigenic relatedness value of less than 5%. When samples cf infected calf faeces were titrated in hyperimmune antisera to these two serotypes, four untypable rotaviruses were isolated of which three belonged to a hitherto unknown third serotype, while the fourth strain showed a partial relationship to this new serotype. In summary of the 108 calf rotavirus strains examined, no fewer than JO (74%) including the UK(Compton), Northern Ireland, and Lincoln strains belonged to serotype 1, one was identified as serotype 2 and three as serotype 3. Of the remainder, one had a partial relationship to serotype J while 23 produced insufficient fluorescing foci to enable them to be characterised fully. Attempts to develop ELISA and HAI tests for the letection of rotaviruses of serotypes 1 and 2 in diagnostic faecal samples croved unsuccessful. Examination of the electrophoretypes of rotavirus involved in 30 outbreaks of diarrhoea in calves revealed that one strain usually predominated in each individual herd, although infection with multiple strains occurred on 5 of the farms examined. There was no evidence that jenomic and polypeptide variations exhibited by the calf rotaviruses were related to their serotypic differences. In experimental calf, infection of ligated gut loops showed that variations in virulence were obtained with different strains of rotavirus, ilthough no correlation occurred between virulence and the serotypes used, fhere was no appreciable difference between faecal and tissue culture virus in the: ability to infect intestinal epithelial cells and most rended to favour the ileal (middle and distal) rather than the jejunal (proximal) parts of the small intestine for replication. The practical importance of indentifying rotavirus serotypes was :onfirmed by passive cross-protection immunisation experiments in jnotobiotic lambs. Thus lambs fed with antiserum for three successive lays and challenged with rotavirus between the first and second feeds showed good protection against the homologous virus; but there was no pross-protection; affected lambs excreted the challenge virus at similar :itres and over the same period as did unprotected control lambs.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Veterinary sciences & veterinary medicine