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Title: The effects of factors influencing human oocyte maturation upon fertilization and preimplantation embryo development
Author: Cavilla, Jennifer Louise
ISNI:       0000 0001 3525 4551
Awarding Body: University of Warwick
Current Institution: University of Warwick
Date of Award: 2002
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The competence of oocytes to mature and undergo fertilization and embryonic development is known to be influenced by the conditions under which their maturation occurs. Suboptimal maturation in vitro currently limits the use of immature oocytes for embryo creation. This project examines the relationship between the conditions of in vitro maturation of human oocytes and aspects of their subsequent developmental competence through the in vitro creation and analysis of research embryos. This work is essential in defining effective and safe conditions for the use of human immature oocytes in programmes of clinical treatment to alleviate infertility. Human immature oocytes were exposed in vitro to various concentrations of meiosis activating sterol (FF-MAS), an endogenous mediator of follicle and oocyte function, or epidermal growth factor (EOP), in the absence of other hormonal support. Their survival and further development relative to controls were measured by assessing the proportions maturing, fertilizing by sperm injection (ICSI), and cleaving in vitro. Image analysis was used to measure various dimensions of oocytes and embryos daily. A pilot study of chromosome and spindle configurations at meiotic metaphase was also conducted. The major findings of this project are that FF-MAS supplementation of maturation medium has different positive effects upon immature oocytes arising from patient groups having different endocrine profiles and yielding differing oocyte populations. FF-MAS at 30J.lg/ml promotes survival of oocytes from unstimulated patients with polycystic ovaries (p
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: QP Physiology ; RG Gynecology and obstetrics