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Title: Fission yeast cell polarity
Author: Monteagudo, Juan Ignacio Mata
ISNI:       0000 0001 3621 0331
Awarding Body: University of London
Current Institution: University College London (University of London)
Date of Award: 1997
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Fission yeast cells are cylindrical. New cell wall materials are deposited only at the poles of the cell, in such a way that cells elongate while keeping both ends directly opposite each other. Mutants in the teal, teal and ban5 genes select growing sites which are not exactly at cell ends. As a result, the poles of the cell are no longer kept opposite, resulting in the production of bent cells and, in more extreme cases, in the formation of branches. The aim of the experiments described in this thesis was to understand the mechanisms by which fission yeast cells select growth sites at the ends of the cell. To do this, I have characterised cytologically and genetically mutants in the teal, teal and ban5 genes, and I have carried out a molecular analysis of the teal gene. The initial study of the mutants revealed abnormalities in the cytoskeleton and cell cycle dependencies in the appearance of the mutant phenotype. To carry out a molecular analysis of teal, the gene was cloned using positional cloning. Both deletion and overexpression of the gene cause a cell shape defect in which cells bend and branch. Antibodies raised against the teal protein were used to determine the intracellular localisation of teal, teal is located at the cell poles Microtubules are continuously required to transfer teal to the cell ends, and teal is located at the ends of microtubules growing towards the cell poles. I propose that teal acts as an end marker, directing the growth machinery to the cell poles. Teal is down-regulated in cells treated with pheromone, which grow towards a mating partner and no longer maintain their ends exactly opposed, teal may also influence microtubular organisation affecting the maintenance of a single central axis.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Genetics