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Title: Immunoglobulin synthesis in leukaemia, lymphoma and immunodeficiency and correlation with normal lymphocyte function
Author: Hannam-Harris, Anne Christine
Awarding Body: University of Southampton
Current Institution: University of Southampton
Date of Award: 1981
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The synthesis and secretion of immunoglobulin (Ig) by cultured B cells from human neoplasia, immunodeficiency and normal lymphoid tissue has been investigated. The Ig light chain (LC) and heavy chain (HC) composition of the secreted material was studied in relation to stages of normal B cell maturation. Secretion of LC in large molar excess over HC was associated consistently with neoplastic lymphocytes demonstrating characteristics of immature cells. In two cases of chronic lymphocytic leukaemia (CLL), secreting free LC, pokeweed mitogen (PWM) stimulation, in vitro, induced balanced chain synthesis, confirmed by the use of anti-idiotypic antibody for precipitation. Normal lymphoid tissue was investigated for the counterparts of these free LC secreting cells. Adult peripheral blood lymphocytes synthesised balanced proportions of Ig chains. Spleen cells, however, demonstrated excess LC production associated with sub-populations of small and intermediate sized cells, probably representing early stages of maturation. As in the CLLs, PWM stimulation induced balanced LC and HC synthesis. Sub-populations of large cells, including mature plasma cells, synthesised balanced Ig. Secretion of free LC, as the predominant Ig product, was demonstrated in some cord blood lymphocyte cultures and in all cultures of liver cells from foetuses of more than 16 weeks gestation, where Ig synthesis was measurable. Two cultures of foetal cells also synthesised free p HC. Free LC secretion occurred in some cases of hypogammaglobulinaemia and related individuals with selective serum IgA deficiency. The synthesis and secretion of free LC appears to be associated predominantly with immature lymphocytes in normal adult tissue; in ontogeny; in immunodeficiency; and in neoplastic transformation. These findings are discussed in relation to current concepts of normal B cell development.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Medicine