Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243523
Title: Genotypes of hepatitis B and C viruses in Nigeria
Author: Oni, Ayodele Oluropo
ISNI:       0000 0001 3459 2268
Awarding Body: University of London
Current Institution: University College London (University of London)
Date of Award: 1996
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Abstract:
A small scale epidemiological survey of hepatitis B and C virus infection amongst healthy adult blood donors and children of pre-school age in Nigeria was carried out using various serological markers of hepatitis B virus (HBsAg, anti-HBs, HBeAg, anti-HBe), and hepatitis C virus infection (anti-HCV ELISA and RIBA tests). Between 10 and 11 % of the adults, and 16% of the children were HBsAg positive. A viraemia rate of 23.8% was found among the HBsAg positive adults, and 25% among the HBsAg positive children. Evidence of past exposure (anti-HBs) among adult blood donors was 41.24%. The subtype of HBV present in Nigeria was determined by amplifying and sequencing HBV DNA, encoding the antigenic determinant a and surrounding region. Two subtypes of HBV are present in Nigeria, adw & ayw. The subtype ayw was in the majority (7/10 of HBV sequenced). A multiple infection with both subtypes of HBV was found in one donor, another donor was infected with both wild type and a variant of ayw. HBV DNA encoding the pre-core and part of the core region of 7 donors was sequenced. One donor was infected with a precore mutant and another was infected with a further variant of HBV. The prevalence of anti-HCV detected by a second generation ELISA was 8% (16/200) of the adult donors, but most of them were negative on supplementary testing. None of the children tested was anti-HCV positive. 25% (4/16) of the anti-HCV positive sera were RIBA test positive and 12.5% (2/16) were indeterminate. Four RIBA positive samples and one negative were HCV-RNA positive, thus 31.25% (5/16) of the ELISA-positive sera were HCV RNA positive by reverse transcriptase polymerase chain reaction (RT-PCR). Three regions of HCV were sequenced, the 5'untranslated region (5'UTR), core and NS5. At least two HCV genotypes are present in Nigeria, type 1 and type 4. Phylogenetic analysis and pairwise comparison confirmed two new subtypes which were provisionally designated 1d and 4i.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.243523  DOI: Not available
Keywords: Blood donors; Phylogenetic analysis; HBV DNA
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