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Title: Identification of phytoplankton pigments from absorption and reflectance spectra
Author: Gómez, Raúl Aguirre
ISNI:       0000 0001 3399 5671
Awarding Body: University of Southampton
Current Institution: University of Southampton
Date of Award: 1996
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The optical properties of open ocean waters are mainly governed by the water itself and phytoplanktonic algae. As contemporary sensors develop higher spectral resolution, there is potential for a greater wealth of information relating to the absorption capabilities of phytoplankton which could help in identifying the major differences in taxonomic groups. Absorption spectra of photosynthetic algae are characterized by a continuous envelope reflecting the strong interaction among pigment molecules. The continuous nature of the spectra makes it difficult to estimate the contribution by each pigment to the total absorption spectra, unless these can be suitably decomposed. Mathematically this task can be accomplished using derivative and spectral deconvolution analyses. This study establishes a methodology for identifying phytoplankton pigments from absorption and reflectance spectra by combining the derivative and spectral deconvolution methods and interpreting the results with knowledge of the distribution of the major light absorbing pigments. For achieving this objective several steps were performed: Firstly, the absorption peaks present in the spectra of different algal groups, measured in laboratory and in situ, were located through the derivative analysis; secondly, a combination of Gaussian-Lorentzian shaped bands, centered at these peaks, were fitted to the original spectra and finally, in situ absorption peaks were related to those of derivative reflectance spectra. Pigment analysis using High Performance Liquid Chromatography (HPLC) provided the biological background for identifying these bands and peaks. The results of this study show that the derivative method can successfully be used to identify pigments by their individual absorption peaks. A good agreement has been found between the original spectra and the reconstructed spectra which were Gaussian-Lorentzian combinations for laboratory and in vivo samples. Analysis of reflectance spectra through absorption spectra also showed promising results. As before, the pigment analysis by HPLC supported the biological interpretation of the bands.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Oceanography