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Title: The role of the major histocompatibility complex in cellular interactions
Author: Birkby, Caroline A.
ISNI:       0000 0001 3465 1280
Awarding Body: University of Glasgow
Current Institution: University of Glasgow
Date of Award: 1983
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1. Endothelial-like cells from murine lung cultures were shown to possess many endothelial - like properties. They appeared similar morphologically to Pig aortic endothelia, had similar intermediate filament proteins, stained positive for Factor VIII antigen, released large amounts of angiotensin converting enzyme, and transmission electron microscopy showed that the cells contained intracellular organelles indicative of an endothelial lineage. 2. Endothelial-like cells showed heterogeneity of H-2Kk expression. This was detected using complement - mediated cytotoxicty techniques in repeated exposure experiments and also by immunofluorescence staining of cell surface H-2Kk . This finding supports other work which demonstrated heterogeneity of H-2 expression in other tissue types. (Eskinazi et al, 1981). 4. Cells obtained from the peritoneal cavity adhere with greater spread areas but in reduced numbers when added to allogeneic H-2D fragment - coated substrates as compared to synoeneic H-2D coated substrates. 5. Peritoneal exudate cells spread out more and in increased numbers when pre-incubated in allogeneic, as opposed to syngeneic, conditioned medium. This effect was shown to be attributable to differences within the H-2K and / or I-A reion of the H-2 complex, since matching at these loci alone has no effect on spread area and counts as compared to fully synqeneic counterparts. Mismatching of PEC's and conditioned media at H-2 K and / or I-A leads to increased counts and spread areas as compared to the effects of syngeneic conditioned media. These increases are significant at the P = 0.05% level of the standard t Test. 6. PEC's adsorb allogeneic I-A fragments from PEC or spleen cell conditioned medium onto their surfaces in an antigenically-recognisable form. Syngeneic I-A fragment binding was not detected by this technique. These results support a new model of cell interactions which has recently been proposed by Curtis (1983a). 7. Preliminary experiments have suggested that when endothelial-like cells of different H-2 types are mixed in aggregation cultures, they may sort out according to H-2 type such that one type surrounds the other in a typical "sphere-within-a sphere" arrangement synonymous with that seen in histotypic sorting out (Steinberg, 1964). This requires further investigation. It is unlikely that H-2 is directly involved in the cell interactions which occur during aggregation since aggregates of similar diameter were obtained with various mixtures of H-2 matched / mismatched cells present in the aggregating mixture. This finding supports previous observations on the collection of H-2 disparate cells to preformed aggregates (McClay and Goodinq, 1978).
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Medicine