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Title: A study on the control of interferon production in lymphoblastoid cells
Author: Baker, Phillip Nigel
ISNI:       0000 0003 9865 4506
Awarding Body: University of Warwick
Current Institution: University of Warwick
Date of Award: 1980
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This research was aimed at increasing the yield of lymphoblastoid interferon produced by the Namalwa line of lymphoblastoid cells. The parameters involved in the induction and production of interferon were studied first to give basic biochemical information about interferon and viral production in Namalwa cells. Optimised conditions were used for the remaining studies. The Namalwa cell line was cloned from single cells to determine whether there was any variation in interferon production in a population of cells. No significant variation was found so a study was made with cells treated with 5’-bromodeoxyuridine and butyric acid. These chemicals alter gene activity in many other cell types. The study was made using the mechanism of superinduction as a basic model for the control factors involved in interferon production. Neither chemical showed identical biochemical effects to the anti-metabolites used to effect superinduction in fibroblast cells. Thus it appears that the two chemicals act in a different manner to anti-metabolites. Neither compound affected the replication of the inducing virus, further investigation showed that 5'-bromodeoxyuridine required incorporation into cellular DNA before increased interferon yields were obtained. The effect of butyric acid on interferon yields could not be directly associated with any of its varied biochemical and morphological effects. These results have increased our knowledge of lymphoblastoid cells and indicated that interferon production in these cells may be controlled in a similar, though not identical way, to the model proposed for fibroblast interferon from fibroblasts. Two chemicals have been shown to increase the yield of interferon from Namalwa cells but the biochemical basis of their activity has not been resolved.
Supervisor: Not available Sponsor: Science Research Council ; Wellcome Trust
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: QR Microbiology