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Title: Studies of vitamin A in nutrition and cancer
Author: Al-Saleh, Maysoon Ahmed Saad
ISNI:       0000 0001 3408 4685
Awarding Body: University of Glasgow
Current Institution: University of Glasgow
Date of Award: 1986
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This thesis describes the development of specific assays for separation and extraction of vitamin A (retinol) and its metabolites, retinal, retinoic acid, retinol acetate, retinol palmitate and its pro-vitamin carotene. The assay employed a liquid/liquid extraction procedure which gave reproducible recoveries for retinol and its metabolites. This was followed by a high pressure liquid chromatography (HPLC) procedure using a silica column with hexane:tetrahydrofuran:formic acid as mobile phase. This gave excellent chromatographic efficiency and resolution of metabolites and resulted in a highly sensitive and specific assay for all the compounds analysed. The assay was used for serum, and was modified to be applicable also for liver and nutritional solutions. A major benefit of this assay compared with previous methods is that all retinol metabolites can be separated on a single column without requirement for expensive gradient systems.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Biochemistry