Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.235471
Title: Immunological aspects of Theileria annulata infection in cattle with reference to the role of the major histocompatibility complex
Author: Innes, Elisabeth Ann
ISNI:       0000 0001 3586 734X
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1989
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Abstract:
An introduction and review of the relevant literature concerning immunological aspects of Theileria annulata infection and the bovine major histocompatibility complex is given as a background to the experimental work presented. An immunisation trial was conducted in Morocco using T. annulata infected lymphoblastoid cell lines (LCL) and the effects of cell dose, cell line and bovine lymphocyte antigen (BoLA) compatibility between cell line and recipient were assessed. All the factors examined were shown to play a role in the response to LCL immunisation although none appeared to be of critical importance. Four schizont infected cel^ cul^ture^ at passage three were inoculated at four different cell doses (10 , 10 , 10 and 10 ) into pairs of calves, one of each pair being BoLA class I matched to the immunising LCL, the other BoLA class I mismatched. The recipients showed variation in the severity of disease symptoms not linearly correlated with the cell dose. The most; severe disease symptoms recorded after inoculation of the LCLs were in the 10 ' cell dose group. Both BoLA class I matched and mismatched LCLs infected and immunised susceptible cattle, although in general the mismatched recipients showed a more^severe reaction. This difference was most marked at the highest cell dose, 10 . One of the four LCLs appeared to be more pathogenic than the other three. The development and specificity of cytotoxic cells generated in vivo in cattle injected with either autologous or allogeneic LCL or sporozoite stabilate material were examined. In cattle immunised with allogeneic LCL at a dose of 10 cells the animals showed mild disease symptoms and the initial cytotoxic response appeared to be directed against the foreign MHC antigens of the LCL, similar to a graft rejection phenomenon. After parasite transfer from LCL to recipient cells and also after a subsequent sporozoite infection and recovery the cytotoxic response was parasite species specific and predominantly MHg restricted to autologous BoLA class I antigens. The recipients of 10 autologous infected cells and the recipients of the sporozoite material showed low levels of non-specific cytotoxic activity after primary immunisation coinciding with severe disease symptoms. After sporozoite challenge, to which both groups were immune, they showed a cytotoxic response similar to that observed in the allogeneic infected cell line recipients after sporozoite challenge. A further study was conducted to examine the target cell for infection in vitro by sporozoites of T. annulata and T. parva. The results suggested that T. annulata preferentially infected cells expressing MHC class II antigens which include B cells and macrophages, whereas T. parva preferentially infected T cells. These findings, if representative of the situation in vivo, may have important implications in the differing pathogenesis of and ease of cell line immunisation against T. annulata and T. parva infections.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.235471  DOI: Not available
Keywords: Bovine Theileria infection
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