Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.726494
Title: Vascular and antithrombitic effects of omega-3 fatty acids in man
Author: Din, Jehangir Nasim
ISNI:       0000 0004 6425 355X
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2014
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Abstract:
Background: Dietary intake of omega-3 fatty acids is associated with a reduction in cardiovascular events. The mechanisms for this are uncertain and previous studies investigating effects on vascular function and thrombosis have produced inconsistent results. We aimed to study the effect of omega-3 fatty acids on endothelial vasomotor function, endogenous fibrinolysis and platelet activation in man. Methods: Firstly, we assessed the effect of dietary intervention with fish rich in marine-derived omega-3 fatty acids on platelet activation in 14 healthy volunteers, using flow cytometric analysis of platelet-monocyte aggregates. Secondly, we investigated the effect of dietary intervention on both platelet-monocyte aggregates and arterial stiffness in a single-blind randomised crossover trial with 30 healthy volunteers. Thirdly, we performed double-blind randomised controlled trials to assess the effect of dietary supplementation with omega-3 fatty acid capsules on endothelial function, endogenous fibrinolysis and platelet-monocyte aggregates in 20 healthy smokers and 20 patients with stable ischaemic heart disease. Endothelial function was assessed using venous occlusion plethysmography during intra-arterial infusions of Substance P, acetylcholine and sodium nitroprusside. Endogenous fibrinolysis was assessed by measuring stimulated release of tissue plasminogen activator (t-PA) from the forearm vasculature during substance P infusion. Results: Dietary intervention with fish rich in marine-derived omega-3 fatty acids reduced platelet-monocyte aggregates in healthy volunteers (16.0±9.0% versus 24.8±10.9%, P < 0.01). Dietary intervention with walnuts rich in plant-derived omega-3 fatty acids did not affect platelet-monocyte aggregates or arterial stiffness in healthy volunteers. Supplementation with omega-3 fatty acid capsules increased endothelium-dependent vasodilatation (P=0.032) and stimulated t-PA release (P < 0.01) in healthy smokers. There was no effect on platelet-monocyte aggregates. Supplementation with omega-3 fatty acids did not affect endothelium-dependent vasodilatation, t-PA release or platelet-monocyte aggregates in patients with stable ischaemic heart disease. There was a marked increase in plasma concentrations of marine-derived omega-3 fatty acids during dietary intervention with oil-rich fish and supplementation with omega-3 fatty acid capsules (P < 0.0001). Discussion: We have demonstrated that dietary intervention with oil-rich fish reduces platelet-monocyte aggregates in healthy volunteers. Dietary intervention with walnuts did not have any effect on platelet-monocyte aggregates or arterial stiffness. Supplementation with omega-3 fatty acids improved endothelial function and endogenous fibrinolysis in healthy smokers, but had no effect on platelet-monocyte aggregates. Omega-3 fatty acids did not affect endothelial function, endogenous fibrinolysis or platelet-monocyte aggregation in patients with ischaemic heart disease.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (M.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.726494  DOI: Not available
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