Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.723610
Title: Progressive changes in the properties of bone during soft tissue decomposition
Author: Walden, Steven J.
ISNI:       0000 0004 6425 6726
Awarding Body: Cardiff University
Current Institution: Cardiff University
Date of Award: 2017
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Abstract:
Changes in bone characteristics during soft tissue putrefaction were investigated over 140 days, equating to between 638 and 1450 cumulative cooling degree days (CCDD) depending on ambient temperature using a porcine experimental model in surface and burial depositions. The hypothesis that changes observed in bone characteristics during soft tissue putrefaction could be utilised for possible forensic applications was proved. Human bones were tested for comparison. The techniques used were colorimetric analysis of staining, measurement of micro-crack lengths (in the order of 0.1 to 1.0 mm) on fractured bone surfaces under scanning electron microscopy, inductively coupled plasma optical emission spectroscopy elemental profiling, thermogravimetric analysis (TGA), zoological mass spectrometry profiling non-collagenous peptide content, and Vickers hardness testing. The findings pertaining to the experimental porcine bone samples were as follows. Stain colour did not equalise between periosteal and fractured cortical bone surfaces. The fracture is widely considered perimortem if said surfaces are homogeneous in colour and postmortem if different. Observed inconsistences in colour change limit the potential of this technique as a potential forensic test of postmortem interval (PMI). After 28 CCDD, shorter intersecting micro-cracks changed to longer linear micro-cracks tracking lamellae. A longitudinal to tangential Vickers hardness (HV) ratio of 1.5 to 1 associated with minimal decomposition indicated 250 CCDD or less elapsed. The same ratio associated with marked decomposition indicated 1450 CCDD or more elapsed. A ratio of less than 1:1 indicated 250 to 1450 CCDD. Decreases in iron, sodium and potassium concentrations associated with tissue fluids can determine if bone is in the early stages of decomposition. TGA correlation of water loss between 22 and 100˚C with observed changes in micro-crack lengths, HV, and elemental profiles suggested progressive dehydration as the underlying common factor. These techniques demonstrated some potential to be developed as forensic tests of PMI. As no correlation with PMI was evident with proteomic profiling of non-collagenous peptides, no such potential was demonstrated.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.723610  DOI: Not available
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