Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.720464
Title: Translating genetics of oomycete resistance from Arabidopsis thaliana into Brassica production
Author: Fairhead, Sebastian
Awarding Body: University of Warwick
Current Institution: University of Warwick
Date of Award: 2016
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Abstract:
White blister rust caused by the obligate pathogen Albugo candida is infectious across the Brassicaceae, and is an economically important disease of cultivated Brassica species. The advance in genotyping technologies has made possible the understanding and deployment of host resistance to plant pathogens, previously unachievable through conventional plant breeding. In this work, the application of Genotyping by Sequencing (GBS) and Resistant Gene Enrichment Sequencing (RenSeq) has identified a single GDSL lipase as a candidate for recessive race non-specific resistance to A. candida in B. oleracea. A second locus has been identified conferring dominant race specific resistance to an A. candida isolate collected from Australia. Much work has been achieved in understanding the genetic basis of resistance to A. candida in the model organism Arabidopsis thaliana, including the identification of white rust resistance (WRR)4, a single dominant resistance (R) gene conferring resistance to A. candida races 2, 4, 7 and 9 in A. thaliana Columbia. In this thesis research, three Columbia-virulent isolates were characterised that are capable of breaking WRR4-Col mediated resistance. Two of these were used to map a new broad spectrum resistance locus, designated WRR4-OyC1, in the vicinity of WRR4 in the Norwegian A. thaliana accession Oy-0 and two additional minor effect QTLs. All three isolates were used for association genetic analysis of genome-wide ‘effectorome’ sequencing to identify candidate genes for avrWRR4 in A. candida for both WRR4, and Oy-0 recognition. From the combined results of this research, a potential strategy for durable white rust control in oilseed and vegetable Brassica would be stacking of at least two R alleles (WRR4-Col and WRR-OyC1) in a genetic background containing the recessive, resistance allele of the GDSL lipase.
Supervisor: Not available Sponsor: Midlands Integrative Biosciences Training Partnership ; Biotechnology and Biological Sciences Research Council
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.720464  DOI: Not available
Keywords: QK Botany
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