Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715570
Title: The development of novel diagnostic sensors based on linear dichroism spectroscopy
Author: Little, Haydn Andrew
Awarding Body: University of Birmingham
Current Institution: University of Birmingham
Date of Award: 2017
Availability of Full Text:
Access from EThOS:
Full text unavailable from EThOS. Thesis embargoed until 31 Dec 2018
Access from Institution:
Abstract:
Within this thesis the use of linear dichroism (LD) spectroscopy was utilised as a new platform for the development of diagnostic sensors. To develop a novel diagnostic sensor in combination with LD spectroscopy a particle with a high aspect ratio is need. Such a particle is M13 bacteriophage, this micron long molecular scaffold is easily alignable in shear flow generating a large LD signal. Using LD spectroscopy, it was possible to demonstrate the successful detection of multiple DNA targets, and that the sensor can also discriminate between DNA sequences differing in length, with a limit of detection (LOD) of 2 M, competitive with the current non amplification methods of DNA detection. The reversibility and regeneration of the sensor were also investigated. Finally, the development of an M13 bacteriophage sensor for the detection of proteins was designed in an unprecedented plug-and-play format. The assay was designed to detect thrombin, which is important in the monitoring of blood clotting disorders. LD spectroscopy enabled the detection of thrombin with a LOD of 10 pM and with a dynamic range from 10 pM to 47 nM.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.715570  DOI: Not available
Keywords: QD Chemistry
Share: