Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.713801
Title: 3-phosphoglycerate dehydrogenase as target in cancer therapy
Author: Unterlass, Judith Edda
Awarding Body: Newcastle University
Current Institution: University of Newcastle upon Tyne
Date of Award: 2016
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Abstract:
Cancer cells adapt their metabolism to simultaneously fulfil the requirements of energy production and biomass generation necessary to sustain high proliferation rates. This deregulated energy metabolism and the proteins responsible therefor provide a potential new route of targeting cancer that has not been thoroughly explored. 3-Phosphoglycerate dehydrogenase (PHGDH), which takes 3-phosphoglycerate (3-PG) out of the glycolytic pathway and into serine production, has been reported as potential target in certain breast cancer forms and melanoma. There is no known inhibitor of PHGDH to date to fully validate the target. Inhibition of PHGDH was explored in breast cancer and melanoma cell lines using siRNA and shRNA interference techniques. Greater knockdown was achieved by siRNA resulting in better growth inhibition than when using shRNA expressing cell lines. The substrate-binding pocket was investigated with substrate analogues and substrate-containing compounds. NAD+-binding was found to be stabilised by coordinated binding of substrates. The catalytic subunits of human PHGDH were crystallised and revealed a flexible lid domain that moves in response to substrate binding. The NAD+-fragment adenosine 5’-diphosphoribose (ADPR) was shown to be a moderate inhibitor of the enzymatic activity of PHGDH and was used for assay validation. Cofactor analogues with different substituents around the pyridine ring were equally suitable to promote the oxidation of 3-PG. A fragment screen was performed using differential scanning fluorimetry and hits were subsequently validated by competition isothermal titration calorimetry. To investigate the fragments in crystals of human PHGDH, a truncated form of PHGDH (construct 93) was engineered by limited proteolysis. Soaking of fragments into crystals of 93 confirmed binding of seven fragments. Structure-activity relationship studies were initiated around the confirmed hits.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.713801  DOI: Not available
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