Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.712072
Title: Characterisation of cello : an ENU-induced mouse model of early-onset hearing loss
Author: Chessum, Lauren
ISNI:       0000 0004 6062 5328
Awarding Body: University of Oxford
Current Institution: University of Oxford
Date of Award: 2015
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Abstract:
cello is a recessive mouse model of early-onset, sensorineural hearing loss that was identified from a large-scale N-ethyl-N-nitrosourea (ENU) mutagenesis screen undertaken at MRC Harwell. Linkage analysis and whole-genome sequencing identified that cello mice have a non-synonymous point mutation in Ikaros family zinc finger 2 (Ikzf2), which encodes a zinc finger transcription factor called helios. To investigate the role of helios in the auditory system, cello mice underwent comprehensive molecular, phenotypic and functional characterisation. The cello mutation was found to disrupt a highly conserved, zinc-coordinating residue in the final C-terminal zinc finger domain of helios. In vitro analyses demonstrated that mutant helios can still localise to the nucleus, but has an impaired ability to homodimerise - a prerequisite for DNA binding. In both Ikzf2+/+ and Ikzf2cello/cello cochleae, helios is expressed in the nuclei of the outer hair cells (OHCs) from the first postnatal week, coinciding with a critical period of OHC functional maturation. Although Ikzf2cello/cello exhibit severely impaired hearing at postnatal day (P) 16, cochlear morphology appears grossly normal up to one month of age, after which the OHCs and inner hair cells begin to progressively degenerate. Transcriptome profiling was subsequently undertaken to identify potential target genes of helios in the auditory system. Results suggested that loss of helios function leads to dysregulation of over 450 genes in Ikzf2cello/cello cochleae at P8. This included the OHC-specific genes Slc26a5 (which encodes the OHC motor protein, prestin) and Ocm (oncomodulin), both of which were significantly decreased in Ikzf2cello/cello mutants compared to Ikzf2+/+ controls. Electrophysiological studies demonstrated that Ikzf2cello/cello OHCs fail to acquire full electromotility, a property that is fundamental to their function, but otherwise mature normally. Using luciferase reporter assays, wild-type helios was found to induce transactivation of the Slc26a5 promoter, whilst this ability was lost in the mutant protein. Additional in silico network analyses identified helios as a putative direct regulator of 40 genes that are enriched in the OHCs and upregulated during development, which suggests that helios has a key role in regulating the maturing OHC transcriptome. Overall, the work presented in this thesis identifies Ikzf2 as a novel hearing loss-causing gene and establishes that the helios transcription factor is essential for OHC functional maturation in the mammalian auditory system.
Supervisor: Bowl, Michael ; Brown, Steve ; Brockdorff, Neil Sponsor: Medical Research Council
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.712072  DOI: Not available
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