Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.699673
Title: Characterization of salivary proteins from New World sandflies
Author: Taha, Ziad Mohamed Deeb
Awarding Body: Keele University
Current Institution: Keele University
Date of Award: 2010
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Abstract:
In the present study, sandfly salivary proteins of Lutzomyia longipalpis were characterised by SDS-PAGE, immunoblotling and MS-based proteomics. Salivary gland extracts (SGE) protein content females was determined, analysed by SDS-PAGE which revealed ~27 peptide bands (range: 5 kDa to 110 kDa), ~33% more than previously reported. Also for the first time, male SGE proteins were characterized by SDS-PAGE which separated ̴14 peptide bands (from ̴12 kDa to ̴105 kDa) constitnting a complex but distinct profile to that obtained for females. Only ̴6 peptide bands were shared between males and females. Sugar feeding did change SDS-PAGE profiles of SGEs from males and females. Total amount of salivary proteins increased with female Sandflyage although salivary proteins were not investigated upon blood-feeding. Comparative SDS-PAGE salivary protein profiles from females of three different siblings of Lutzomyia longipalpis showed reproducible differences. Immunoblotting of Lutzomyia longipalpis siblings’ SGE proteins against sera from hamsters that have been repeatedly challenged with only one of the siblings revealed a number of sibling-specific of salivary antigens.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.699673  DOI: Not available
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