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Title: In vitro osteoclast resorption of calcium phosphate bone substitutes
Author: Martin, Joanne
ISNI:       0000 0004 5990 6384
Awarding Body: Queen's University Belfast
Current Institution: Queen's University Belfast
Date of Award: 2015
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Resorption of calcium phosphate (CaP) biomaterials is traditionally assessed using an osteoclast (QC) resorption assay where resorption pits formed on the CaP surface are analysed by microscopy techniques and quantified on the basis of pit number, pit area or pit volume. Pit area measurements (20) have become common practice when assessing CaP biomaterial resorption in vitro. Apart from the time consuming nature of pit area analysis techniques it is not a precise indicator of resorption; variations in pit depth are not taken into consideration and it is unsuitable for use on porous materials where visualisation of internal structures is difficult or for use on materials with rough surfaces where determination of individual pits would be difficult. A 3D quantification of bioresorption is available but requires specialised, expensive equipment. An appropriate measure of resorption was required to be more efficient and more cost-effective than the current available in vitro methods, but most importantly, to directly correlate with pit area measurements and have the ability to be used in a broad range of in vitro experiments. An QC resorption assay was developed and optimised through a series of experiments. The established assay was used to assess several outcome measures as potential indicators of resorption in vitro, namely; the correlation of percentage area resorbed in vitro with Ca and P concentration in cell culture medium, QC number and QC activity. A dense substrate free from surface anomalies was used to accurately correlate pit area with the alternative outcome measures. This body of work has established two main outcome measures of bioresorption in vitro that correlate with pit area measurements; Ca and P ion concentration in culture medium and QC specific enzyme activity. These outcome measures will prove invaluable for improving the fundamental understanding of QC resorption of CaP biomaterials.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available