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Title: Functional genomic studies of selected Eurasian frogs
Author: Lyu, Peng
ISNI:       0000 0004 5990 547X
Awarding Body: Queen's University Belfast
Current Institution: Queen's University Belfast
Date of Award: 2015
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Amphibians have existed on Earth for more than 300 million years. Most amphibians can survive in a wild range of living environments. Some of their living environments are fulfilled with pathogen microorganisms. The skin of amphibians produces a complex noxious/toxic chemical cocktail from specialised granular glands to against these microorganisms. This thesis describes several novel bioactive peptides which were identified from the skin secretions of the Chinese Bamboo odorous frog, Odorrana versabilis, the Chinese Black-spotted Pond frog, Pelophylax nigromaculatus and the Eurasian edible frog, Pelophylax kl. esculentus, by using reverse phase HPLC and molecular cloning. The first discovery in this study was ornithokinin, obtained from the skin secretion of Odorrana versabilis. In addition, a novel Bowman-Birk like trypsin proteinase inhibitor peptide with strong myotropic activity, named QUB 1813, was isolated from the skin secretion of the Eurasian edible frog, Pelophylax,kl. esculentus. A novel antimicrobial peptide, esculentin-2Blm, was then isolated from the skin secretion of Pelophylax kl. esculentus. These novel antimicrobial peptides show dramatic antimicrobial activities but with associated relatively high haemolytic activities. Finally, a novel myotropic peptide, named QUB 1067, was obtained from the skin of the Chinese Black-spotted Pond frog, Pelophylax nigromaculatus. QUB 1067 has a completely novel primary structure and exhibits contractile activity on isolated rat uterus smooth muscle tissues. All of these bioactive peptides were synthesised by employing PS3 solid-phase Fmoc chemistry. The synthetic replicates were used in the bioactive assays, such as antimicrobial activity assays, haemolytic assays, smooth muscle assays and trypsin inhibitory assays.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available