Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.695657
Title: Functional genomic studies on the skin secretions of selected Peruvian frogs
Author: Li, Yihan
ISNI:       0000 0004 5990 539X
Awarding Body: Queen's University Belfast
Current Institution: Queen's University Belfast
Date of Award: 2015
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Abstract:
Amphibian skin secretion has been studied for over fifty years and has been considered as a treasure trove of an extraordinarily-rich variety of biologically-active compounds. Novel peptides are constantly being discovered in amphibian skin secretions and some have counterparts in mammals, thus it is commonly thought that their study is an excellent way for the discovery of new mammalian peptides. Model genomic and proteomic techniques have been applied in this study to identify and characterise several gene-encoded peptides from selected Peruvian frogs, Phyllomedusa baltea, Phyllomedusa palliata and Phyllomedu'sa tomopterna. Two novel phylloseptin peptides and three different dermaseptin peptides were identified, and their synthetic replicates exhibited different levels of antibacterial activities. Both phylloseptin and dermaseptin peptides are representative members of peptide families identified from phyllomedusine frogs. A novel dodecapeptide, which was named FR-12, was found to possess dose-dependent relaxation effects on rat ileum smooth muscle. Moreover, specifically-designed analogues showed different potencies as compared with the natural peptides and the comparisons obtained revealed that the structural parameters of antimicrobial peptides are interdependent and jointly determine their activities.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.695657  DOI: Not available
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