Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.695221
Title: 3-epi-25 hydroxyvitamin D : assay development and measurement
Author: Bennett , Sarah
Awarding Body: Queen Margaret University
Current Institution: Queen's University Belfast
Date of Award: 2015
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Abstract:
Vitamin D is a prohormone produced in the skin following ultraviolet B (UVB) radiation exposure. It can also be obtained from natural sources such as oily fish, as well as from fortified foods or supplements. The main physiological role of vitamin 0 is in the maintenance of skeletal health. However, there is also emerging evidence that vitamin 0 may also be associated with several non-skeletal health outcomes including; type 2 diabetes mellitus (T2DM), cardiovascular disease (CVD) and decreased pregnancy complications. Suboptimal vitamin 0 status is a common global issue. The accurate measurement of 250HD is hindered by epimers. Epimers are isomers which only differ in the configuration at one carbon atom. Subsequently, they have the same molecular weight, and can overlap chromatographically with vitamin 0 metabolites or internal standard peaks and give false estimates of true 250HD levels. In the present study, an ultra-performance liquid chromatography tandem mass spectrometry (UPLC/MS-MS) assay was developed, which was capable of assessing 250HD and 3-epi-250HD concentrations in human serum/plasma. The UPLC/MS-MS assay was used to measure 250HD and 3-epi-250HD levels in two cohorts; the first cohort consisted of pregnant women with type one diabetes and pregnant controls, while the other was a nested case-control cohort from the Prospective Epidemiological Study of Myocardial Infarction (PRIME) study, which consisted of men who had suffered a CVD event and controls. These results were then used to determine potential relationships between 250HD and 3-epi-250HD concentrations and; gestational and pregnancy outcomes, BMI, cardiovascular disease risk, as well as seasonal effects.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.695221  DOI: Not available
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