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Title: Candidate biomarkers of renal warm ischaemia in a donation after circulatory death large animal model
Author: Hossain , Mohammad Ayaz
ISNI:       0000 0004 5918 8159
Awarding Body: St George's, University of London
Current Institution: St George's, University of London
Date of Award: 2014
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Background The increased use of donation after circulatory death (DCD) kidneys in the UK has followed a UK government mandate of a 50% increase in organ donation from 2008- 2013. DCD kidneys have associated higher rates of delayed graft function (DGF) and primary non-function (PNF), which is thought to be due to warm ischaemia (WI) exposure during the retrieval process. This project aimed to utilise a porcine model of DCD WI in order to examine candidate biomarkers. Both a proteomic (2D DIGE) and a genomic (expression microarray) study were undertaken with appropriate validation. Methods Schedule 1 termination of six large white pigs with intravenous phenobarbitone was followed by open renal biopsies taken at 30 min intervals up to 180 min. Total RNA and proteins were extracted and subjected to single colour expression microarray and 2D difference in gel electrophoresis (20 OIGE) respectively. Validation of the proteomic and genomic studies was performed with Western Blotting and quantitative RT-PCR (qRT-PCR) respectively. Results Upregulation of HSP70 was found to be significant across the three hour WI period at the gene (Fold change (FO) +4.1, p
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available