Use this URL to cite or link to this record in EThOS:
Title: Investigating the interactions between plant viruses and host stomata
Author: Murray, Rose Rebecca
ISNI:       0000 0004 5917 9818
Awarding Body: University of Bristol
Current Institution: University of Bristol
Date of Award: 2015
Availability of Full Text:
Access from EThOS:
Stomata are microscopic pores located in the epidermis of most terrestrial plants. As well as serving as gateways for gas exchange and transpiration, stomata have recently become known to have increasingly complex relationships with pathogens, with many pathogens utilising stomata as entry portals. However, the research so far has largely focussed on bacterial and fungal pathogens, leaving a gap in the knowledge about the interactions between plant viruses and stomata. This study aims to investigate the interactions and relations between stomata and viruses by investigating potential for stomata to act as entry portals for viruses and the developmental changes which occur under virus infection. In order to test the hypothesis that stomata can act as entry portals for plant viruses, a series of experiments was performed which altered stomatal apertures before applying purified virus suspended in solution in an aerosol. It was found that Nicotiana tabacum plants were more likely to become infected when TMV virus solution was applied when stomata were open. Natural and chemical factors were used to manipulate stomatal apertures prior to virus application. Stomatal development was investigated following a virus infection. A range of host-virus systems was used and it was found that susceptible host types had a general reduction in stomatal index and density when infected with a virus. Transcripts of genes involved in stomatal development were also tested for changes in healthy and infected plants and were found to vary upon infection. Knock-out mutants of various stomatal developmental or functional genes showed varying developmental responses to a virus infection, with notable changes in rin4 which resulted in an increase in stomatal development post infection. The results presented in this project provide an insight into a relatively new field of research which has so far been neglected in the field of plant pathology.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available