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Title: Advances in anaesthetics for fish : greater understanding of species specific welfare associated with induction and efficacy
Author: Readman, Gareth D.
ISNI:       0000 0004 5917 1744
Awarding Body: University of Bristol
Current Institution: University of Bristol
Date of Award: 2015
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There is very little information within the scientific literature on the aversiveness of anaesthetic agents for fish, as well as a paucity of information on the actual efficacy of the agents used to ameliorate the pain associated with surgery in fish. The report of the Animal procedures committee (2009), concluded that urgent work was necessary in order to assess the aversive nature of anaesthetic agents, for fish. Initial assessment of anaesthetic agents used a chemotaxic chamber to quantify behavioural response to commonly used anaesthetics in zebrafish, carp, fathead minnow, medaka and rainbow trout. Investigations show that for zebrafish, the commonly used anaesthetic agents MS222 and benzocaine elicited a significant aversive response, however, for tribromoethanol (TBE) and etomidate no significant differences were demonstrated between the time spent in the exposure versus control lanes. For carp, MS222 and benzocaine did not elicit a significant response, however, etomidate was found to be aversive. Medaka showed significant aversion to MS222 and benzocaine but as with zebrafish no significant response to etomidate, TBE however wasn't tested against medaka. Rainbow trout showed no version to MS222, bezocaine or etomidate. It was not possible to quantify the potential aversion of fathead minnows to different anaesthetic agents due to the inability to generate a positive control. Differences in the behavioural response to anaesthetic agents between species signifies the need for species specific care and welfare for fish. Further work investigated the development of a method of EEG recording in carp. Changes in EEG total power (Ptot) and visual evoked potentials (VEPs) in relation to induction and maintenance of anaesthesia with either MS222 or etomidate and the application of noxious stimuli were quantified. No significant difference was seen in changes in Plot as a result of noxious stimulus, demonstrating the ability of both agents to achieve a plane of surgical anaesthesia.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available