Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.685183
Title: Development of polymers for nanoreactors and enzyme stabilisation
Author: Farrance, Katherine
ISNI:       0000 0004 5924 1984
Awarding Body: University of Warwick
Current Institution: University of Warwick
Date of Award: 2016
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Abstract:
This thesis explores the use of polymer nanoreactors in catalysis as well as the use of water-soluble polymers in enzyme stabilisation. Chapter 1: An introduction to the literature currently covering the research topics relevant to the techniques used within this thesis, providing further background on the fundamental enzyme theory that is to be explored Chapter 2: This looks at the techniques available for tethering the catalyst triazabicyclodec-5-ene to the hydrophobic block of an amphiphilic block copolymer, synthesised by nitroxide mediated polymerisation and the assembly of these polymers. Chapter 3: The previously synthesised nanoreactors were used to catalyse Michael additions with varied loadings within the core of the micelles. Chapter 4: Looks into the effect of using water soluble polymers of varying molecule weights on the activity of Lipases Circular dichroism spectroscopy and stain tests were used as tools to indicate changes to the native structure and activity. Chapter 5: Thermostability of lipase was investigated, and the use of the water-soluble polymers to extend the lifetime of the enzyme in solution was investigated through monitoring changes in the melting temperatures as well as stability studies.
Supervisor: Not available Sponsor: University of Warwick ; Unilever
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.685183  DOI: Not available
Keywords: QD Chemistry
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