Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.682966
Title: Delayed bolting in rocket for improved quality and greater sustainability
Author: Taylor, Jemma Louise
Awarding Body: University of Warwick
Current Institution: University of Warwick
Date of Award: 2015
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Abstract:
Wild rocket (Diplotaxis tenuifolia) has recently become a popular salad leaf in the UK due to its peppery taste. It is grown widely in Italy but is now being produced in Spring and Summer in England. It is part of the Brassicaceae family and thus has a high level of homology at the DNA level to other Brassica species and Arabidopsis. This project aims to produce late bolting genotypes of rocket to incorporate into commercial breeding programmes. Delayed bolting is important as current varieties flower at unpredictable times and often earlier than desired. This is a problem because when rocket flowers it becomes unsaleable. Ethyl methanesulphonate (EMS) was used to generate a mutant population of rocket. Late bolting lines were selected and whole genome sequencing was used to identify single nucleotide polymorphisms (SNPs) induced by the EMS which may be a cause of the late bolting phenotype. Six flowering pathway genes have been isolated from rocket and have been tested to see if they can functionally complement A. thaliana knockout lines in these genes. Further work was carried out to investigate how these genes were expressed over diurnal and developmental time courses to understand their function in the flowering time pathway in rocket. Together, these results show that most of the flowering pathway genes isolated from rocket are functional orthologues of those in Arabidopsis. The photoperiodic and vernalization requirements of rocket were investigated and it was found that rocket does not have a vernalization requirement and is a facultative long day plant. Targeted mutagenesis using clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR associated system (cas9) was employed for the introduction of mutations into the FLOWERING LOCUS T (FT) gene in rocket and Arabidopsis. Many late flowering Arabidopsis lines were identified and sequencing revealed the successful manipulation of the FT gene. Two late bolting rocket lines were also identified. Overall, the project aims were achieved as late bolting rocket lines have been produced and will be incorporated into a rocket breeding programme.
Supervisor: Not available Sponsor: Wellcome Trust ; Elsoms Seeds Ltd
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.682966  DOI: Not available
Keywords: SB Plant culture
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