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Title: Molecular basis of herbivore resistance in Brassica napus
Author: McInnes, Kirsty Jamie
ISNI:       0000 0004 5921 9963
Awarding Body: University of Glasgow
Current Institution: University of Glasgow
Date of Award: 2015
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Oilseed rape (Brassica napus) is a commercially important agricultural crop susceptible to damage from invertebrate herbivores, such as caterpillars, aphids and slugs. Plants can detect the presence of invertebrates via physical contact, tissue consumption, and on recognition of compounds in saliva. Plant retaliation includes the production of proteinase inhibitors to impair gut function and the accumulation of phenylpropanoids and potentially toxic glucosinolates to decrease plant palatability. Previous work has shown that a component of sunlight, ultraviolet-B (UV-B) radiation, can regulate defence related responses in a manner similar to that of pests and the plant wound-response hormone, Jasmonic acid (JA). The molecular basis behind UV-B- enhanced plant defence against invertebrates, however, remains elusive. This project aims to better understand invertebrate resistance in oilseed rape along with the genetic and metabolic basis of UV-B-enhanced defence against two agricultural pests, the grey field slug and caterpillars of the Diamondback moth (Plutella xylostella). UV-B treatment of B.napus and Arabidopsis thaliana has been found to enhance their resistance to these pests, and gene expression analysis of B.napus identified several genes similarly regulated by UV-B radiation, JA application, and/or slug or Plutella grazing. It is thought that these genes are important in UV-B enhanced plant resistance. Transgenic Arabidopsis lines over-expressing three of these oilseed rape genes have been generated to evaluate their role in UV-B-mediated defence. If found to be more resistant to pests, these lines will serve as ‘proof of concept’ that manipulation of the UV-B response pathway in members of the Brassica family could be used to develop new invertebrate resistant varieties.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: QR Microbiology ; SB Plant culture