Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.680380
Title: Genetic transformation of red macroalgae : using novel techniques on the commercially important species Chondrus crispus
Author: Ramessur, A. D.
ISNI:       0000 0004 5915 4637
Awarding Body: Queen's University Belfast
Current Institution: Queen's University Belfast
Date of Award: 2014
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Abstract:
The macroalgae, or seaweeds, are ecologically and evolutionarily important but the progress of molecular research in seaweeds has been seriously hampered by the lack of transformation methodologies. One restriction is that tools which allow transformation in land plants such as biolistics and Agrobacterium have often not been tested or optimized in seaweeds. Accordingly, my thesis will use the recently sequenced genome of Chondrus crispus to develop transformation techniques in this seaweed. For biolistics, bombardment was optimised using the commonly used GUS expression cassette, pCAMBIA 1301; these bombardment parameters were subsequently used to test the expression of a native Chondrus promoter construct of my own design. Findings from biolistics were extended to test/present evidence that Chondrus thalli are also amenable to infection by Agrobacterium. Successful GUS localisation was viewed by histochemical staining within cortical and medullary cells.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.680380  DOI: Not available
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