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Title: Spatio-angular microscopy
Author: Kielhorn, Martin
Awarding Body: King's College London
Current Institution: King's College London (University of London)
Date of Award: 2013
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Photobleaching and phototoxicity pose a problem in live cell imaging. Fluorescence imaging induces reactive oxygen species in observed organisms which can alter the behaviour of the sample. Hence, minimising the light exposure is an important goal. We augment a widefield epifluorescence microscope with two spatial light modulators. By controlling the spatial excitation pattern and the angle of illumination, we can adapt the illumination to the specimen. In many cases, this technique will create exposures with reduced excitation of the out-of-focus fluorophores, resulting in better image quality and less phototoxicity. My custom software is used to obtain an initial image stack of the specimen. Subsequent image sections are exposed with excitation patterns that account for the previous image stack. Depending upon the distribution of fluorophores, this adaptive exposure can considerably reduce photobleaching and phototoxicity.
Supervisor: Heintzmann, Rainer Walther ; Suhling, Klaus Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available