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Title: Identifying transplanted cells in the context of regenerative medicine for stroke
Author: Nicholls, Francesca Joan
ISNI:       0000 0004 5368 2965
Awarding Body: King's College London
Current Institution: King's College London (University of London)
Date of Award: 2015
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Exogenous labels are often used to identify transplanted cells in order to elucidate their therapeutic mechanisms. In the context of stem and progenitor cell transplants, the requirements for exogenous labels must be ever more stringent in order to avoid both short and long term effects on the biology of these sensitive cell types, as well as to ensure accurate identification of transplanted cells. As cell therapies become more complex, there is also a need to be able to simultaneously image different cell populations in vivo. Here four commonly used “optical” labeling protocols (Hoechst, BrdU, PKH26 and Qtracker) and an MRI contrast agent (a new Gadolinium nanoparticle based on ProHance) are assessed for their effects on human neural stem cell (hNSC) biology and their sensitivity and reliability for identification of transplanted cells. With a view to developing useful paradigms for in vivo imaging of tissue engineering, two ParaCEST (Paramagnetic Chemical Exchange Saturation Transfer) agents are used to label hNSCs and human brain microvascular endothelial cells. MRI is then used to image each cell population separately after transplantation into the stroke cavity in a rat MCAO (middle cerebral artery occlusion) model. These studies will allow the selection of labels that will have minimal effects on NSC biology whilst retaining maximal label reliability, and thereby allow accurate elucidation of the effects of complex cell therapies.
Supervisor: Williams, Brenda Patricia Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available