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Title: Development and application of platform technologies for the study of lipid phase behaviour and biomembrane mechanics
Author: Purushothaman, Sowmya
Awarding Body: Imperial College London
Current Institution: Imperial College London
Date of Award: 2012
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This thesis presents the design, development and application of a number of platform technologies for the study of lipid-phase behaviour and bio-membrane mechanics. This includes an automated laboratory based X-ray beamline with a multi-capillary sample chamber capable of undertaking small angle X-ray scattering measurements on a maximum of 104 samples at a time as a function of temperature between 5 and 85 C. The modular format of the system enables the user to simultaneously equilibrate samples at eight different temperatures with an accuracy of +/-0.005 C. This system couples a rotating anode generator and 2-D optoelectronic detector with Franks X-ray optics, leading to typical exposure times of less than ve minutes for lyotropic liquid crystalline samples. Beamline control including sample exchange and data acquisition has been fully automated via a custom designed LabVIEW framework. In addition this thesis presents an overview of the development of a suite of tools for undertaking fluctuation analysis measurements of lipid vesicles under a variety of conditions including as a function of hydrostatic pressure. These and other biophysical techniques have been used to study a variety of binary lipidic systems determining key parameters ranging from spontaneous curvature and bending rigidity through to p-T dependent phase behaviour.
Supervisor: Ces, Oscar Sponsor: Biotechnology and Biological Sciences Research Council
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available