Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.675536
Title: Do myosins contribute to metastasis of prostate cancer cells?
Author: Makowska, Katarzyna Anna
ISNI:       0000 0004 5371 4066
Awarding Body: University of Leeds
Current Institution: University of Leeds
Date of Award: 2015
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Abstract:
Prostate cancer is the second most common cause of death from cancer in men in the UK. Localised disease can be treated with surgery or radiotherapy, but metastasis remains a great therapeutic challenge. Cancer cell migration involves rearrangements of the actin cytoskeleton, which is mediated by its interaction with myosins, a large and diverse family of motor proteins involved in many processes crucial for cell migration, such as cell adhesion, cell polarity or endocytosis. It is likely that the activity of myosins contributes to metastatic spread. I investigated the myosin expression profile in prostate cancer cell lines and found that Myo1b, Myo9b, Myo10 and Myo18a were expressed at higher levels in cells with high metastatic potential. Using an siRNA-based approach, knockdown of each myosin resulted in distinct phenotypes. Myo10 knockdown drastically decreased filopodia of PC3 cells, Myo18a knockdown increased filaments of non-muscle myosin 2A, knockdown of Myo1b and Myo9b increased stress fibre formation. Loss of Myo10 affected cell migration in 2D. In all cases, cell spread area was increased and 3D migration potential was decreased for Myo1b, Myo10 and Myo18a. Myo1b, Myo10 and Myo18a were also expressed in benign prostatic hyperplasia although knockdown of these myosins in benign tissue did not have very clear effects. Glioblastoma cells expressed high levels of Myo10 and showed decreased protrusions after Myo10 knockdown. Taken together, myosins act as molecular motors but also directly influence actin organisation and cell morphology and migration, which can contribute to the metastatic phenotype of cancer cells.
Supervisor: Peckham, Michelle ; Wells, Claire Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.675536  DOI: Not available
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