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Title: The environment in Tanzania as a source of Mycobacterium tuberculosis complex and diversity analysis of the slow growing mycobacteria
Author: Hung, Ju-Jiun
ISNI:       0000 0004 5371 2060
Awarding Body: University of Warwick
Current Institution: University of Warwick
Date of Award: 2015
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Tuberculosis (TB) is the most prevalent infectious human disease and health burden worldwide. There are over eight million incident cases of TB and this has caused more than one million deaths globally. In addition, bovine Tuberculosis (bTB) has become widespread with the disease endemic in most African countries, including Tanzania. For this reason, detection and treatment for the two causal agents Mycobacterium tuberculosis (Mtb) and Mycobacterium bovis (Mb) has become a priority in Tanzania. Other slow growing mycobacteria (SGM) are also responsible for respiratory disease in humans and animals. This project focuses on the environment as a potential reservoir of Mtb, Mb and other SGM by comparison of samples from different sites and use of case controls which can help to establish if shedding correlates with disease and indeed can be a potential source of continuing infection. In this study, IMC-qPCR and different specific primers RD4 scar and RD9 were developed to detect and quantify Mtb and Mb in a range of environmental samples taken during the dry and wet season from villages in Tanzania. Mtb and Mb were both detected in cattle faecal samples taken from animals kept by the same household. For the SGM prevalence and diversity specific primers were used for oligotyping following deep sequence analysis by pyrosequencing of amplicons. The oligotyping result matched the identification of bacterial species in cattle lesion and wildlife tissue. The higher prevalence of Mtb was detected from households with TB patients compared to control households. This work is part of a collaboration with UCD and USF in USA and SUA in Tanzania so data on infection status of human and animal populations will be made available following approvals then compared with environmental reservoirs.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: RC Internal medicine