Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.673967
Title: Preliminary studies on the genetics of bacteriophages capable of generalized transduction
Author: Hedges, R. W.
Awarding Body: University of Leicester
Current Institution: University of Leicester
Date of Award: 1969
Availability of Full Text:
Access through EThOS:
Access through Institution:
Abstract:
Theoretical arguments are presented to indicate that bacteriophages capable of generalized transduction are also capable of forming composite genetic structures containing DNA sequences from the bacteriophage genome and also DNA from other genetic elements. These modified bacteriophage genomes - comparable with ? dg genomes should be capable of effecting transduction. Various techniques for isolating such modified bacteriophage genomes are discussed all of which require that transduction be effected under conditions in which the transduced fragment cannot be integrated into the genome of the recipient. Transduction of strains which have suffered large deletions, of strains that have lost (by mutation) the ability to undergo recombination, of fragments of an episome into a strain not carrying an homologous episome and of chromosomal genes between different bacterial genera. Attempts to isolate modified prophages by all four techniques are described. The only experiments that lead to well defined and regularly repeated were experiments in which bacteriophage ? 15 (or a mutant of this bacteriophage deficient in ability to effect antigenic conversion) was grown on a strain of Salmonella anatum carrying an F' factor integrated into which was a segment of DNA derived from the chromosome of Escherichia coli and selection was made among the recipients for those which had acquired genetic markers from the E. coli DNA. In the early experiments the lactose operon of an Flac episome was the genetic marker transduced. Among the transductants were strains carrying defective ? 15 prophages which had suffered loss of various genetic markers. Some of these transductants were immune to superinfection: others were not. Some of the trans-ductants were effective lysogens producing HFT lysates. Since the Salmonellae have no lactose operon, transduction of these genes is a special case. Studies on transduction of the Escherichia arabinose operon gave encouraging preliminary results. Some experiments concerning genetic instability are also described.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.673967  DOI: Not available
Share: