Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.670393
Title: The phytoalexin response in Phaseolus vulgaris L.
Author: da Cunha, Antoinette
Awarding Body: University of Oxford
Current Institution: University of Oxford
Date of Award: 1986
Availability of Full Text:
Access from EThOS:
Full text unavailable from EThOS. Please try the link below.
Access from Institution:
Abstract:
This thesis concerns the initial events leading to the induction of the phytoalexin response in the Phaseolus vulgaris L. - Colletotrichum lindemuthianum L. host - pathogen interaction The phytoalexin response is an expression of the resistance of the host to further pathogen invasion. The visible expression of the phytoalexin response is hypersensitive necrosis. One of the initial events leading to the induction of the phytoalexin response is thought to be the induction of the first enzyme leading to the synthesis of phenylpropanoid phytoalexins, namely L-phenylalanine ammonia-lyase (PAL). Standard methods for determination of PAL activities were found to lead to measurements of both phenylalanine amino-transferase (PAT) and PAL activities together. Further, PAT was found to have a higher affinity for L-phenylalanine than PAL. An isotopic assay was devised for the accurate estimation of PAT and PAL activities separately using a specific inhibitor of PAT, L-aspartic acid. These experiments were carried out in a whole (intact) plant system. A rapidly isolated cell and protoplast system wa.s specially devised for isolation of the pathogen cell wall constituents responsible for the induction of the phytoalexin response. Special care was taken to minimise mechanical damage in these systems by optimising methods of purification, viability and intactness. The pathogen cell wall component responsible for the induction of the phytoalexin response was found to be an asialoglycoprotein.
Supervisor: Whatley, F. R. Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.670393  DOI: Not available
Keywords: Phytopathogenic microorganisms
Share: