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Title: Peptide based hydrogels in the study of mesenchymal stem cells for the purposes of regenerative medicine
Author: Miller, Angela
Awarding Body: University of Glasgow
Current Institution: University of Glasgow
Date of Award: 2015
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Regenerative medicine is a vastly expanding subject area, with a number of different strategies and substrates being studied to ultimately create a model for repairing diseased and injured tissue. Stem cells are a promising cell type in this field as they are known to differentiate into a number of cell types and contribute to normal cell repair. However, despite their potential as a useful cell choice in the field of regenerative medicine, stem cell based therapies have limited potential due to their ability to form tumours when implanted in the human body, problems arising with immunogenicity and the difficulty in obtaining adequate cell numbers for transplantation. To overcome these problems, many research groups are interested in using biomimetic substrates and scaffolds to mimic the architecture, chemical composition and stiffness properties of the in vivo cell niche and various human tissues, but in an in vitro setting. By doing so, the MSC behaviour can be studied and the differential lineages examined allowing the desired substrate to be tuned to obtain the desired cellular outcome. In this work, hydrogels composed of Fmoc diphenylalanine and Fmoc Serine have been characterised and used to study changes in Mesenchymal stem cell (MSC) responses in a two and three dimensional state. The results obtained from this work demonstrate that such hydrogels support MSC growth and produce a mixed phenotype population which differentiates over time. A further hydrogel paired with collagen has shown promise in promoting MSC differentiation down the osteogenic lineage and has potential for the future study in maxillofacial reconstruction models.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Q Science (General) ; QH301 Biology