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Title: Interfacial phenomena between bacterial or mammalian cells and orthopaedic biomaterials
Author: Preedy, Emily Callard
ISNI:       0000 0004 5368 7117
Awarding Body: Cardiff University
Current Institution: Cardiff University
Date of Award: 2015
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Adhesion as a scientific phenomenon has been researched for the past 70 years, as the notion of two entities contacting effects a huge expanse of daily activities, from writing to sophisticated cellular and bacterial interactions essential for growth and survival. Inherently, a robust and adequate model of adhesion was acquired, one in which biological aspects were considered. Initially, the methodology required was optimised using the atomic force microscope (AFM) by testing a model bone substrate against ultra-high molecular weight polyethylene (UHMWPE), a material commonly found in the articulating acetabular cup. Once a force mapping technique was established experimentation continued to bacterial adhesion against model bone samples of various roughness, establishing that the adhesion phenomena occurs at a scale dependency due to the alterations in the topography of the surface at the micro to nano level. Aseptic loosening and osteolysis are major causes of failures in implanted biomedical devices at the hip. These issues are governed by the deterioration of the moving components, producing particles known as wear debris associated with the metals, bone cement, and UHMWPE materials initiating an immune response which is detrimental to the surrounding cells and tissues adjacent to the implant. The notion of mechanical aspects altering the health of mammalian cells has been ignored throughout the research of implantations and their effect on the cells by foreign bodies; the only concept studied to date is the viability and functionality post exposure. Therefore, this thesis aims at observing ii mesenchymal and osteoblast (both rodent and human) cells associated to wear debris (metal and polymeric particles of various sizes and compositions) exposure and the effect this has on cell nanomechanical and adhesive properties using the AFM techniques. The data obtained indicated that Cobalt nanoparticles were more damaging on all cell types than Titanium and polymeric particles.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: RM Therapeutics. Pharmacology ; TA Engineering (General). Civil engineering (General)