Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.666582
Title: Regulation of senescence in Arabidopsis thaliana and barley
Author: Comadira, Gloria
ISNI:       0000 0004 5355 4007
Awarding Body: University of Leeds
Current Institution: University of Leeds
Date of Award: 2015
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Abstract:
Senescence is the last stage of leaf development. During leaf senescence essential nutrients are remobilised to the growing parts of the plant. This highly regulated process involves the expression of senescence-associated genes and a decrease in photosynthesis accompanied by chloroplast protein degradation. The following studies were performed in order to characterise the functions of the papain-like cysteine proteases and the WHIRLY1 protein in the regulation of senescence. Transgenic Arabidopsis lines expressing the rice cystatin, oryzacystatin-I (OCI) in either the chloroplasts or cytosol were characterized. These lines had a slow growth phenotype relative to the wild type. Furthermore, ectopic OCI expression in Arabidopsis increased leaf numbers and enhanced shoot and root branching at flowering. Transgenic barley lines with decreased levels of WHIRLY1 transcripts were grown under optimal and low nitrogen stress conditions. Under optimal conditions, the WHIRLY1 transgenic lines had similar CO2 assimilation rates to those of the wild type but they had significantly more chlorophyll and less sucrose. Moreover, these lines produced fewer tillers and seeds than the wild type. The WHIRLY1 transgenic plants grew better than the wild type under nitrogen deficiency, maintaining higher photosynthetic capacity and chlorophyll content. The WHIRLY1 protein influenced the expression of specific subsets of transcripts encoding chloroplast proteins including thylakoid NADH and cytochrome b6/f complexes. Additionally, the transcript profile revealed new target proteins for WHIRLY1, supporting the involvement of this protein in chloroplast signalling and stress responses. Finally, this study allowed the successful production of barley plants expressing SAG21 and wheat plants expressing the OCI gene in the cytosol, which have the potential to improve stress tolerance and increase seed yield.
Supervisor: Foyer, Christine Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.666582  DOI: Not available
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