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Title: Identification of potential marker proteins of toxicant-induced damage to spermatogenesis
Author: McLaren, Tanya Thomson
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1994
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Spermatogenesis involves a complex series of cell-cell interactions which are probably mediated by secreted proteins. The primary objective of the studies described in this thesis was therefore to identify specific proteins which change in relative abundance in the early stages of toxicant-induced damage to spermatogenesis and which might have potential use as markers of such damage. Identification of such proteins might pin-point the possible biochemical causes of the toxic effects on the testis, and also give insight into normal control mechanisms in spermatogenesis. The chemicals used in these studies were meta-dinitrobenzene (m-DNB), nitrobenzene (NB) and methoxyacetic acid (MAA). The effect of severe disruption of spermatogenesis, induced by short-term local testicular heating, was assessed in order to establish whether protein changes comparable to those seen following toxicant exposure could be identified. Within 4 hours of treatment, stage-specific changes in the incorporation of 35S-methionine into both secreted proteins and intracellular proteins were deserved. Analysis by 2-D SDS PAGE identified 8 proteins which were affected adversely following heat treatment, all of which had been affected by toxicant exposure. In conclusion, the studies presented in this thesis have identified proteins which have potential use as markers of early toxicant-induced damage to spermatogenesis. Studies to date in the rat have identified proteins in peripheral blood which derive from the Sertoli cells and germ cells and the expectation is that most if not all ST-secreted proteins will appear in blood. Therefore the logical next step will be to determine whether any of the proteins identified in the present studies are detectable in peripheral blood and whether the amounts change following toxicant exposure.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available