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Title: Mesenchymal stem cells and the ocular surface
Author: Branch, Matthew James
Awarding Body: University of Nottingham
Current Institution: University of Nottingham
Date of Award: 2013
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Interest in Mesenchymal Stem Cells for ophthalmic regenerative medicine is increasing. These cells have an increasing array of abilities that allow them to promote wound healing through a number of different mechanisms. The area of mesenchymal stem cell research is large and complex, with many differing names, criteria, sources and culture techniques. Thorough characterisation is crucial if they are to be developed for therapeutic use. Research into fetal liver mesenchymal stem cells represents a small proportion of what is known about these cells although some evidence points to important differences between those found in the bone marrow of adults. These cells may be able to aid corneal regeneration or provide a suitable model for other mesenchymal stem cells. Detailed characterisation of plastic adherent fetal liver was carried out. These cells successfully met the International Society of Cellular Therapy's minimal criteria for mesenchymal stem cells. Their phenotype and genotype (karyotype and telomere length) were assessed over extended passaging. Marker expression was found to decrease in correlation with telomere shortening and increasing cytogenetic anomalies. Fetal liver mesenchymal stem cell colony forming abi lities were investigated and although colonies proliferated substantially marker profiles in each varied and did not conform to the minimal criteria. Mesenchymal stem cells do not generate an immune response and most are also reported to suppress the cellular immune system. Although fetal liver mesenchymal stem cells did not generate an immune response when incubated with lymphocytes they did not possess the ability to suppress stimulation by third party antigens. Fetal liver mesenchymal stem cell interaction with amniotic membrane, as a potential candidate for a culture substrate and carrier was also analysed. Amniotic membrane allowed attachment and proliferation whilst retaining a mesenchymal stem cell phenotype after 21 days in culture.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available