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Title: Characterisation of phage and phage biology towards development of novel DNA vaccines
Author: Bagheri, Hassan Moeiniyan
Awarding Body: University of Nottingham
Current Institution: University of Nottingham
Date of Award: 2013
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The first use of DNA for vaccination was reported more than 20 years ago. Generally in DNA vaccines, a genetically engineered plasmid DNA is injected into the host cells where it is expressed and produces immunogenic proteins. These proteins are recognized by the immune system and as a result an immune response is initiated. Using DNA vaccines has many advantages over conventional vaccines however there are also some problems and limitation associated with these vaccines. One major problem is in the ability to deliver the DNA to the target cells in the body without it first being degraded. This issue has been addressed using various modifications to the standard delivery system and developing new vehicles for delivering DNA. One example is to use phage vaccination technology, where a DNA vaccine is delivered in a recombinant phage particle. The second limiting step is the translocation of DNA to the nucleus. In eukaryotic cells this is important in determining the efficiency of vaccination. It is still not clear exactly how DNA can enter the nucleus but there are some techniques that have the potential to enhance the delivery of DNA, which is the subject of this study. The strategy we have used here was to investigate the use of modification of the terminal DNA binding protein of phage PRD1 by fusion to a nuclear localization sequence (NLS) to determine whether this could improve the efficiency of DNA translocation. In this study, the terminal protein was successfully cloned, modified and expressed and it was shown that addition of an NLS sequence did not appear to have any effect on the functionality of the DNA binding protein. However the work did not progress far enough to determine its potential impact on DNA translocation this is yet to be determined.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available