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Title: Molecular analysis of a gene affecting a quantitative trait in Drosophila melanogaster
Author: Zur Lage, Petra
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1993
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During an artificial selection experiment by T.F.C. Mackay for high and low abdominal bristle number in lines of Drosophila melanogaster which had been exposed to P element mutagenesis, a new allele of the smooth gene exhibiting an extremely low number of bristles on the abdomen was isolated. The molecular cloning and characterisation of the smooth gene is described here. Genomic cloning of the P element insertion in the new mutant allele was carried out and sequences immediately adjacent to either side of the P element insertion were subcloned. These were used to screen a cDNA library from which a 2.6 kb cDNA clone was isolated. Northern analysis using the cDNA as a probe confirmed the presence of a single transcript of equivalent size showing varying levels of expression during development. The 2.6 kb cDNA clone was completely sequenced and the genomic organisation of smooth was established revealing that the gene consists of ten exons differing in size from 64 bp to 590 bp and introns from 60 bp to more than 20 kb. The smooth gene extends over a genomic region more than 74 kb in length. The predicted gene product is a 53 kD protein that shows a high degree of sequence similarity to a group of RNA-binding proteins and in particular to the human heterogeneous nuclear ribonucleoprotein L. The possible role of smooth in RNA metabolism and the way in which mutations in this gene give rise to alteration in bristle number are discussed.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available