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Title: Characterisation of Invadolysin, a novel essential metalloprotease in Drosophila melanogaster
Author: Yu, Bin
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2007
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The IX-14 mutant was first found as a mutation affecting neuroblasts and imaginal discs and later characterised as a mutant affecting chromosome condensation. Base on its sequence, IX-14 may be classified as a metalloprotease that is a homologue of leishmanolysin. IX-14 have a wide range of functions in cell cycle and cell migration, probably through its alternative spliced forms or its diverse substrates. In this study, I have showed that IX-14 mutants have a chromosome structure defect besides a length-wise chromosome hyper-condensation phenotype. The turnover of three nuclear envelope proteins (lamin Dm0, otefin and lamin A/C) was affected in IX-14 mutants. One of them, lamin Dm0, has been shown to be cleaved by IX-14 in vitro, which suggests it is a substrate of IX-14. I have also shown Drosophila IX-14 mutant embryos have a germ cell migration defect, germ cells failing to form gonads at stage 13 after passing through the midgut. This was consistent with the suggestion that IX-14 might play a migration role based on the observation that IX-14 localized to the leading edge of macrophages and as invadopodia-like structures in some HeLa cells. Due to the presence of a signal sequence at the N-terminus and a GPI anchor sequence at the C-terminus of DmIX-14, I could not purify DmIX-14 since it keeps losing its tags on both the N-terminus and the C-terminus in the baculovirus system. Methods to express large scale active IX-14 still need to be found.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available