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Title: Dual hollow fibre bioreactor for the growth of hybridomas
Author: Wright, Kevin Ian Trevor
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1996
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The use of hollow fibre bioreactors in mammalian cell culture has provided a means by which large populations of viable cells can be grown continuously, for extended periods, in a relatively small volume of vessel. Bioreactors in which one set of fibres are used for nutrient and metabolite exchange from the cell mass are limited by their reliance on an axial flow regime. This pressure mediated flow pattern leads to the formation of nutrient gradients within the cell mass, which occur due to the large diffusional distances present in the growth space, resulting in cell death within the bioreactor. The Edinburgh Dual Hollow Fibre Bioreactor solves this diffusional problem by using two sets of fibres to mimic the arterio-venous flow found in the body. In this instance the diffusional distances are reduced due to the close proximity of the two sets of fibres. Using this design, and a murine hybridoma (ES4) which produces an IgM blood typing antibody, cells were grown to a maximum density of 1.2x107 viable cells ml-1, with a maximum antibody production rate of 0.16 mgh-1. This was a 10 fold improvement in the viable cell number obtained using an airlift fermenter. The successful operation of this design was found to be dependent upon the pressure profiles developed within the bioreactor, with a number of extended culture experiments having been carried out. The influence of bioreactor design, fibre selection and the methods by which these bioreactors should be operated are discussed in this work.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available