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Title: ER to Golgi transport during apoptosis
Author: Walker, Annemieke
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2002
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Newly synthesised proteins are delivered from the ER to the Golgi and finally to storage granules or directly to the plasma membrane. Inhibition of ER to Golgi transport, during mitosis coincides with the fragmentation of the Golgi complex into thousands of vesicles, dispersed throughout the cytoplasm, which can be visualised by indirect immunofluorescence. The Golgi apparatus was poorly resolved in neutrophils by immunofluorescence microscopy. Therefore, a model system in cell lines was used to study Golgi morphology during apoptosis. The data show that fragmentation of the Golgi apparatus occurs during apoptosis in NRK, HeLa and J.CaM1.6 cells using various stimuli to induce apoptosis. In HeLa cells treated with SSP, the Golgi-derived vesicles were demonstrated to be morphologically indistinct to those found in mitotic cells by electron microscopy. GM130, a key Golgi-localised protein involved in tethering incoming secretory vesicles to the cis-Golgi, is known to be reversibly phosphorylated during mitosis, contributing to the inhibition of ER to Golgi transport and the fragmentation of the Golgi complex. GM130 is also shown to be a target during apoptosis, where its cleavage is caspase-dependent. p115, the GM130 binding partner however, is not cleaved during apoptosis. Moreover, the data presented indicate that ER to Golgi transport is inhibited during apoptosis by transfecting COS-7 cells with an expression vector containing the gene for Vescular Stomatitis Virus glycoprotein (VSVG). In untreated COS-7 cells, VSVG was shown to be transported to the cell surface by indirect immunofluorescence. In apoptotic cells however, VSVG was demonstrated to co-localise with the ER marker. The results herein demonstrate an inhibition of ER to Golgi transport during apoptosis. This appears to be a ubiquitous event during apoptosis, providing the first model that explains the environmental isolation of the apoptotic neutrophil.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available