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Title: Synthesis and purification of oligodeoxyribonucleotides
Author: Wahl, Franck Olivier
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1993
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A new highly hydrophobic 5'-hydroxyl protecting group (Tbf-DMTr) has been designed for the purification of synthetic oligonucleotides. Tbf-DMTr-oligonucleotides are strongly retained on RP-HPLC allowing a facile separation from truncated sequences. Subsequently the group can be removed in acidic conditions. The fluorescent properties of TBf-DMTr enable easy detection. The synthesis and purification of long oligonucleotides (> 100-mer) has been undertaken. Additionally a new fluorescent label for oligonucleotides has been developed which enables detection of DNA probes to concentration down to 10-10 mol/l.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available