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Title: Evaluation of cell wall components as potential recognition factors in solanaceous grafts using a model system
Author: Wade-Royle, Elizabeth Mary
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1994
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The Nicandra physaloides/Lycopersicon esculentum heterograft consistently fails to produce functional vascular reconnections across the graft union (GU). Evidence supplied by earlier workers culminated in the hypothesis that a recognition system may exist between stock and scion. This thesis documents attempts to identify the stage [1] "off" signal for cell division in a model GU provided by actively-dividing suspension-cultured cells of L.esculentum x peruvianum and L.esculentum cv. Ailsa Craig (L.esculentum AC) by monitoring protein synthesis. The application of deproteinated cell walls of N.physaloides, L.esculentum AC, and L.esculentum x peruvianum consistently inhibited incorporation of [14C] leucine into protein, as did hemicelluloses from all sources at pH 4.5. pH measurements of apoplastic fluid from the GU of homografts and heterografts demonstrated a minimum value 4 d after grafting. In homografts GU pH increased thereafter, but in incompatible heterografts pH rose and then dropped. [14C]-labelled cell wall components were used to trace the fate of pectins and hemicelluloses applied to suspension-cultured cells; for cells of L.esculentum x peruvianum about 75% of the associated exogenous pectin was ionically bound to the cell surface, while 25% was internalised or bound by non-ionic means. At pH 6.0 about 73% of the associated hemicellulose bound to the cell surface while approximately 27% was internalised, but at pH 4.5 these proportions were almost 100% and 0% respectively. Experiments with cells of L.esculentum AC revealed that at pH 6.0 approximately 44% of the associated hemicellulose had bound to the cell surface, while about 55% had been internalised; when incubation occurred at pH 4.5 these percentages remained almost unchanged. The behaviour of the two types of suspension-cultured cells to identical stimuli is compared, and the relevance of the responses discussed in relation to graft compatibility.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available