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Title: The study of a Hawaiian skirt gene (HWS) suppressor in Arabidopsis and a HWS orthologous gene erect panicle 3 (EP3) in rice
Author: Yu, Hongyang
Awarding Body: University of Nottingham
Current Institution: University of Nottingham
Date of Award: 2014
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Abstract:
Floral organ detachment takes place at a specifically programmed stage during development. However, sepals of the mutant hawaiian skirt (hws-l) fail to shed and are retained until silique desiccation. The mutant is a consequence of a 28bp deletion in the ORF of the F-box gene At3g61590, which is part of one of the three core subunits of the SCF complex of plant ubiquitin E3 ligases (Gonzalez-Carranza et al., 2007). To dissect the role of HWS in regulating plant development, hws-l seeds were mutagenized with EMS and a series of F2 populations were obtained. The 35.1, a suppressor line was identified and studied in detail. The mapping of the 35.1 showed that the mutated gene is located in the APETALAl (At1g69120) gene locus and sequencing analysis showed an amino acid change from Ser to Phe in the conserved MADS box domain. To confirm this observation, a 35.1/hws-l plant was crossed with an ap1 T-DNA insertion line (NASC ID: N651561) and the phenotype of ap1 is observed in the progenies. qPCR was performed to detect expression of genes involved in the hypothesised regulatory network and indicated that HWS may play a role in repressing APl expression. This regulatory network is likely to control sepal boundary formation with a CUCl and CUC2 related regulatory network.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.663258  DOI: Not available
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