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Title: Microsatellite markers in genetic improvement of livestock
Author: Usha, A. P.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1995
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Genome mapping is in the forefront of interest among both plant and animal breeders, enabling the relationship between genes, genome structure and function to be investigated in addition to identifying the location of genes. The prerequisite of a linkage map of the genome is the availability of a large number of highly polymorphic and informative marker loci which are evenly distributed throughout the genome. Microsatellite loci provide the unique class of markers which overcome many of the difficulties associated with the other market types. The availability of a detailed genetic map of the bovine genome could enhance the genetic progress in cattle breeding programmes through the identification of loci affecting traits of economic importance. Other potential applications of genetic markers include their use in confirmation of parentage, individual identification, germplasm evaluation and identification of disease loci. In this thesis, microsatellite markers are investigated in three areas, a) parentage verification and individual identification b) study of phylogenetic relationship and c) mapping a lethal defect in Dexter cattle. a) Five highly polymorphic microsatellite markers CYP21, DRB3, FSHB, ETH131 and HEL6 were evaluated for parentage verification using 275 animals belonging to 15 breeds of cattle. Some breeds were found not be in Hardy Weinberg Equilibrium (HWE), the deviation being greatest in those breeds which had an excess of homozygotes. A new approach was developed for calculating the Probability of RAndom Sire Exclusion (PRASE) taking into account the deviation from HWE and linkage between markers, using observed genotype frequencies. Taken together, the linked markers, DRB3 and CYP1 gave a PRASE of 0.88 in all breeds with success ranging from 0.75-0.96. Including a third marker the PRASE was increased to 0.97, and with all five markers 0.99 or better was achieved for all the 15 breeds.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available